The Fast and Simple LC-MS/MS Method for Determination of Rifampicin in the Human Blood Plasma

Abstract:

Background: Rifampicin is an important anti tuberculosis drug for the early infection and, more importantly, bactericidal activity against mycobacterium tuberculosis. LC-MS/MS, with the required molecular specificity, provides quantitative method to rapidly differentiate between the drugs and their metabolites. Aim: The current study represents the fast and simple LC-MS/MS method for determination of rifampicin in the human blood plasma. Materials and Methods: The plasma samples containing the rifampicin drug were cleaned up by using the protein precipitation method. The chromatographic division was performed by utilizing the ZORBAX Eclipse plus C₁₈ Column (4.6 mm X 150 mm, 5µm). The versatile stage comprised of acetonitrile and 10 Mm ammonium acetic acid derivation in a proportion of 80:20% V/V, wash the column with the blend of solvents comprising of acetonitrile and water 80:20% V/V, maintaining column oven temperature at 30ºC±1ºC and the auto sampler temperature is kept up with at 10ºC±1ºC. The injection volume of the sample is 10.0 µL and the total run time of the experiment is 4 min with a flow rate of 1000 mL/min and with a split ratio of 50:50 for the entire experiment. Results and Discussion: An LC-MS/MS method for the rifampicin from the sample was performed. Sample volume of 0.300 ml, the injection volume is taken as 10.0 µL and total run time is 4 min, RT for rifampicin is 1.30±0.5 min and the reference drug roxithromycin RT is 3.00±0.5 min. Conclusion: The transient LC-MS/MS study permits the assessment of enormous quantities of blood tests in a brief timeframe with fast, simple and successful readiness, giving a quick, dependable and best device for RIF clinical observing and review.

Keywords: Rifampicin, LC-MS/MS, Protein precipitation, Acetonitrile.