Rapid, Cost Effective and Simple Analytical Method Development and Validation for Simultaneous Estimation of Thymol and Cholecalciferol by Ultraviolet Spectrophotometry

Abstract:

Background: Thymol and Cholecalciferol in a combined approach can be used as antioxidant, free radical scavenging, anti-depressant, anti-inflammatory, anti-diabetic, anti-arthritis etc. So, rapid, cost effective and simple analytical method is required to estimate Thymol and Cholecalciferol simultaneously in the mixture. Purpose: The purpose of this work is to develop and validate Ultraviolet spectroscopic (UV) method for simultaneous estimation of Thymol and Cholecalciferol. Materials and Methods: Two methods were developed i.e., simultaneous equation method and Q-Absorbance ratio method. In simultaneous equation method, absorbance of Thymol and Cholecalciferol were measured at λ_max of 276.3 nm and 263.2 nm. While in Q-Absorbance ratio method, absorbance of Thymol and Cholecalciferol were measured at 270.1 nm (isosbestic point) and at 263.2 nm (λ_max of Cholecalciferol). Methanol was used as a solvent in both the methods. Results: The concentration of the individual drugs in the combined mixture was found in the range of 98% to 100% in both methods. Developed methods were validated as per ICH quality guidelines. The validation parameters checked were linearity, range, LOD, LOQ, precision and accuracy. Conclusion: Both the methods were found accurate, simple, precise and reproducible for simultaneous estimation of Thymol and Cholecalciferol. These developed analytical methods can be used for quantification of Thymol and Cholecalciferol in pharmaceutical dosage forms.

 Keywords: Thymol, Cholecalciferol, UV-visible spectroscopy, Simultaneous equation method development, Q-absorbance ratio method development, Analytical method validation.