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Published on:10th Feb 2015
Indian Journal of Pharmaceutical Education and Research, 2010; 44(2):136-141
Pharmaceutical Education | doi:Nill

Demonstration of Cytosine Deoxynucleoside Deamination by the Mutagen Nitrous Acid


Authors and affiliation (s):

M. Ariatti* and A.S. Gupthar

Department of Biochemistry, Faculty of Science and Agriculture, Westville Campus, University of KwaZulu-Natal, Private Bag X54001, Durban, 4000, South Africa

Abstract:

A practical class exercise to illustrate the nitrous acid-induced deamination of cytosine-containing 2'- deoxynucleosides is described. This is suitable for undergraduate students in Pharmaceutics working in a laboratory equipped with a scanning UV spectrophotometer. Students are provided with microlitre amounts of 2'-deoxycytidine and 2'-deoxy-5-methylcytidine solutions, which are treated with sodium nitrite in acetic acid. Products are identified by thin layer chromatography (TLC) and UV spectral comparison with standards. The aim is to impress upon students the ease with which DNA bases containing exo amino groups may undergo deaminations and to highlight the genotypic and possible phenotypic consequences of this reaction in native DNA.

Key Words: cytosine, nitrous acid, mutagen Indian Journal

 




 

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