Characterization, Partial Purification of Alkaline Protease from Intestinal Waste of Scomberomorus Guttatus and Production of Laundry Detergent with Alkaline Protease Additive

Abstract:

Background: Fish industry wastes are an important environmental contamination source. A way of minimizing the environmental problems generated by the high amount of fish wastes is its transformation to a commercially useful ingredient. Alkaline proteases, one of the most important industrial enzymes are extracted especially from the Intestine of fish. They are widely used in laundry detergents for the degradation of protein. Methods: The protease extracted from Seer fish (Scomberomorus guttatus) was precipitated with ammonium sulphate (70%). The enzyme was more stable over a wide range of pH (8-12) and its optimum temperature was around 60°C.The enzyme activity was also analyzed by Zymogram with casein. The enzyme was partially purified using Sephadex G-75. The compatibility of the Enzyme with various commercial detergents was studied. Results: Enzyme showed activity in detergent even after 1 hour of incubation. De-staining activity of the enzyme with various stains such as Blood stain, Food spices, Beetroot stain, raw banana stain etc was studied. Enzyme showed de-staining activity. Thus proving its applications in Laundry industry. Conclusion: By virtue of our findings, it could infer that alkaline protease was successfully produced from the intestinal waste of seer fish. Also, a new laundry detergent with enzyme additive was produced and the de-staining activity of the newly prepared detergent with enzyme additive was promising and thus can be used in places such as hospitals, food industry and households. It can be concluded that not only a valuable enzyme can be produced from a cost free sample but also dumping of the waste be rectified.

.Key words: Protease, Seer Fish, Scomberomorus guttatus, Alkaline proteases, Zymogram, Commercial detergents, Laundry detergent.